AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Manuel d'utilisateur Page 47

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DRAFT
December 16, 2004 3:54 pm, GSG_Chapter_4-04.fm
Chapter 4 Ordering Primers and Probes
Overview
40 Primer Express Software 3.0 Getting Started Guide
Notes
Overview
About This
Chapter
This chapter provides information on how to order your selected primer and probes.
Ordering Primers and Probes
After the Primer Express
®
Software generates the table of candidate primers and probes,
you can order those that best suit your needs.
1. In the Primer/Probe tab, select the primer and probe set you want to order.
2. Click on the Order tab.
3. Click on the toolbar to go the Applied Biosystems online store.
4. Log into the AB Store if you have an account, register if you are a new user.
Ordering Primers 1. Below the ABI PRISM
®
Primers/Probes heading, under the TaqMan Primers and
Probes heading, click Sequence Detection Primers.
2. In the Product Information tab, select the check box next to the volume of primers
to order.
3. Below the primer option you selected, click Customize.
4. Follow the instructions on the web page to specify any options.
5. Follow the instructions on the web page to enter or copy your sequence text.
6. Type the name for the Forward Primer, press Enter, then copy and paste your
primer sequence from either the Order tab or the Primer Probe Test Tool (in the
Primer Express software).
7. Type the name for the Reverse Primer, press Enter, then copy and paste your
primer sequence from either the Order tab or the Primer Probe Test Tool (in the
Primer Express software).
8. Click Continue.
9. Review your order, then click Add to Basket. If this completes your order (SYBR
®
Green Dye assays), click Proceed to Checkout and follow the instructions on the
web page to complete your order. Otherwise, click Continue Shopping to add
Green Dye or probes to your order.
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