AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Manuel d'utilisateur télécharger pdf (Page 27)

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December 16, 2004 3:54 pm, GSG_Chapter_4-02.fm

Chapter 2 Designing Primers and Probes for Quantification Assays

Automatically Designing Primers for SYBR

Green Dye Assays

20 Primer Express Software 3.0 Getting Started Guide

Notes

Figure 5. Sequence tab

Note: If you select the Double-Stranded checkbox in the Sequence tab, both sense and

reverse strands will be displayed. However, primers are designed using the sense strand

sequence only.

Finding Primers To find primers:

Select Tools > Find Primers/Probes ( ). Primer Express software performs its

calculations based on default parameter values.

The status bar, located at the bottom of the window, displays information about the

progress of the calculations as the software searches for primer/probe sets. If primers are

found, go to “Viewing Results” on page 21.

If primers were not found:

If the software does not find primers using default parameters, a pop-up will appear

stating that no acceptable primer pairs were found and that you can see the Interim

Results window. For more information on Interim Results, see Primer Express Software

Version 3.0 Online Help.

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AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Manuel d'utilisateur Page 27