AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Manuel d'utilisateur télécharger pdf (Page 17)

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December 16, 2004 3:54 pm, GSG_Chapter_4-02.fm

Chapter 2 Designing Primers and Probes for Quantification Assays

Automatically Designing Primers and Probes

10 Primer Express Software 3.0 Getting Started Guide

Notes

Figure 2. Sequence tab

Note: If you select the Double-Stranded checkbox in the Sequence tab, both sense and

anti-sense strands will be displayed. However, primers and probes are designed using the

sense strand sequence only.

Finding Primers

and Probes

To find primers and probes:

Select Tools > Find Primers/Probes ( ). Primer Express software performs its

calculations based on default parameter values.

The status bar, located at the bottom of the window, displays information about the

progress of the calculations as the software searches for primer/probe sets. If primers and

probes are found, go to “Viewing Results” on page 11.

If primers and probes were not found:

If the software does not find primers and probes using default parameters, a pop-up will

appear stating that no acceptable primer pairs were found and that you can see the

Interim Results window. For more information on Interim Results, see Primer Express

Software Version 3.0 Online Help.

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AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Manuel d'utilisateur Page 17