AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Manuel d'utilisateur Page 40
- Page / 52
- Table des matières
- MARQUE LIVRES
Noté. / 5. Basé sur avis des utilisateurs
DRAFT
December 16, 2004 3:54 pm, GSG_Chapter_4-03.fm
Chapter 3 Designing Primers and Probes for Allelic Discrimination Assays
Automatically Designing Primers and Probes
Primer Express Software 3.0 Getting Started Guide 33
Notes
3
Figure 12. Probe annotations in Sequence tab
Note: After the software finds primers and probes, the sequence box is locked. To edit
the sequence, click to unlock.
As a general guideline, select the primer/probe sets with a low Penalty score and a low
amplicon length (if the Penalty score and Amplicon Length fields are not displayed,
scroll to the right in the table). However, all primer/probe sets generated using default
parameters meet primer and probe guidelines. For more information regarding Penalty
scores, see Primer Express Software Version 3.0 Online Help.
Saving the
Document
Before proceeding to other designs, be sure to save the Primer/Probe annotations and
results found. Select File > Save As to save the document for future use.
Ordering Primers
and Probes
To order your selected primers and probes, refer to Chapter 4, “Ordering Primers and
Probes.”
IMPORTANT! Before running your samples, Applied Biosystems recommends that you
run control samples to verify the performance of the selected primers and probes.
- Getting Started Guide 1
- Chapter 1 3
- Chapter 2 3
- Chapter 3 3
- Chapter 4 3
- Contents 4
- How to Use This Guide 6
- How to Obtain Support 7
- Before You Begin 8
- Introduction 10
- Installing the Software 11
- Chapter 1 Before You Begin 12
- Quantification Assays 14
- Overview 15
- To load a sequence file: 16
- Finding Primers 17
- Document 19
- Ordering Primers 19
- Saving the 19
- If primers were not found: 22
- To design the Reverse Primer: 24
- Green Dye 26
- Green Dye Assays 27
- Allelic Discrimination Assays 34
- Assigning a SNP 37
- To design the Forward Primer: 44
- Ordering Primers and Probes 46
- Part Number 4362460 Rev. B 52
Produits connexes et manuels pour Téléviseurs et moniteurs AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE
Téléviseurs et moniteurs AMX DESIGN XPRESS-PROFESSIONAL V 1.1 - PROGRAMMER GUIDE Spécifications
(23 pages)
(23 pages)
Téléviseurs et moniteurs AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Guide de l'utilisateur
(288 pages)
(288 pages)
Sony SVS1313ZDZB Handbücher
Bedienungsanleitungen und Benutzerhandbücher für Powerbanks Sony SVS1313ZDZB.
Wir stellen 1 PDF-Handbücher Sony SVS1313ZDZB zum kostenlosen herunterladen nach Dokumenttypen zur Verfügung Schnellstart Benutzerhandbuch
Commentaires sur ces manuels