AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Manuel d'utilisateur Page 1

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Page 1 - Getting Started Guide

Before You BeginDesigning Primers and Probes for Quantification AssaysDesigning Primers and Probes for Allelic Discrimination AssaysOrdering Primers a

Page 2

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-01.fmChapter 1 Before You BeginIntroductionPrimer Express Software 3.0 Getting Started Guide 3Notes1Prim

Page 3 - Chapter 4

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-01.fmChapter 1 Before You BeginInstalling the Software4 Primer Express Software 3.0 Getting Started Guid

Page 4 - Contents

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-01.fmChapter 1 Before You BeginInstalling the SoftwarePrimer Express Software 3.0 Getting Started Guide

Page 5

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-01.fmChapter 1 Before You BeginBasics6 Primer Express Software 3.0 Getting Started GuideNotesBasicsStart

Page 6 - How to Use This Guide

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmPrimer Express Software 3.0 Getting Started Guide 7NotesChapter 22Designing Primers and Probes forQ

Page 7 - How to Obtain Support

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysOverview8 Primer Express Software 3

Page 8 - Before You Begin

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysAutomatically Designing Primers and

Page 9

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysAutomatically Designing Primers and

Page 10 - Introduction

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysAutomatically Designing Primers and

Page 11 - Installing the Software

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysAutomatically Designing Primers and

Page 12 - Chapter 1 Before You Begin

© Copyright 2004, Applied Biosystems. All rights reserved.For Research Use Only. Not for use in diagnostic procedures.Information in this document is

Page 13

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysManually Designing Primers and Prob

Page 14 - Quantification Assays

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysManually Designing Primers and Prob

Page 15 - Overview

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysManually Designing Primers and Prob

Page 16 - To load a sequence file:

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysManually Designing Primers and Prob

Page 17 - Finding Primers

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysManually Designing Primers and Prob

Page 18

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysManually Designing Primers and Prob

Page 19 - Saving the

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysAutomatically Designing Primers for

Page 20

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysAutomatically Designing Primers for

Page 21

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysAutomatically Designing Primers for

Page 22 - If primers were not found:

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysAutomatically Designing Primers for

Page 23

DRAFTDecember 16, 2004 3:56 pm, GSG_Workflow.fmBefore You BeginChapter 1Designing Primersand Probes forQuantificationAssaysChapter 2Designing Primersa

Page 24 - To design the Reverse Primer:

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysManually Designing Primers for SYBR

Page 25

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysManually Designing Primers for SYBR

Page 26 - Green Dye

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysManually Designing Primers for SYBR

Page 27 - Green Dye Assays

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysManually Designing Primers for SYBR

Page 28

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmPrimer Express Software 3.0 Getting Started Guide 27NotesChapter 33Designing Primers and Probes for

Page 29

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmChapter 3 Designing Primers and Probes for Allelic Discrimination AssaysOverview28 Primer Express S

Page 30

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmChapter 3 Designing Primers and Probes for Allelic Discrimination AssaysAutomatically Designing Pri

Page 31

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmChapter 3 Designing Primers and Probes for Allelic Discrimination AssaysAutomatically Designing Pri

Page 32

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmChapter 3 Designing Primers and Probes for Allelic Discrimination AssaysAutomatically Designing Pri

Page 33

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmChapter 3 Designing Primers and Probes for Allelic Discrimination AssaysAutomatically Designing Pri

Page 34 - Allelic Discrimination Assays

DRAFTDecember 16, 2004 3:54 pm, GSG_4tabsTOC.fmContentsPrimer Express Software Version 3.0 Getting Started Guide vPreface viiChapter 1 Before You Begi

Page 35

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmChapter 3 Designing Primers and Probes for Allelic Discrimination AssaysAutomatically Designing Pri

Page 36

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmChapter 3 Designing Primers and Probes for Allelic Discrimination AssaysManually Designing Primers

Page 37 - Assigning a SNP

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmChapter 3 Designing Primers and Probes for Allelic Discrimination AssaysManually Designing Primers

Page 38

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmChapter 3 Designing Primers and Probes for Allelic Discrimination AssaysManually Designing Primers

Page 39

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmChapter 3 Designing Primers and Probes for Allelic Discrimination AssaysManually Designing Primers

Page 40

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmChapter 3 Designing Primers and Probes for Allelic Discrimination AssaysManually Designing Primers

Page 41

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-04.fmPrimer Express Software 3.0 Getting Started Guide 39NotesChapter 44Ordering Primers and ProbesOrder

Page 42

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-04.fmChapter 4 Ordering Primers and ProbesOverview40 Primer Express Software 3.0 Getting Started GuideNo

Page 43

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-04.fmChapter 4 Ordering Primers and ProbesOrdering Primers and ProbesPrimer Express Software 3.0 Getting

Page 44 - To design the Forward Primer:

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-04.fmChapter 4 Ordering Primers and ProbesOrdering Primers and Probes42 Primer Express Software 3.0 Gett

Page 45

vi Primer Express Software Version 3.0 Getting Started GuideDRAFTDecember 16, 2004 3:54 pm, GSG_4tabsTOC.fm

Page 46 - Ordering Primers and Probes

Primer Express Software 3.0 Getting Started Guide 43IndexAadd DNA file 9, 19, 29allele 2, 34, 36, 37allelic discriminationcreating the document 29alle

Page 47

44 Primer Express Software 3.0 Getting Started GuideIndexSNP Target 30SNP target 30SNP Target Tool 31Starting and Exiting Primer Express Software 6SYB

Page 48

Worldwide Sales and SupportApplied Biosystems vast distribution and service network, composed of highly trained support and applications personnel, re

Page 49

DRAFTDecember 16, 2004 3:54 pm, GSG_Preface.fmPrimer Express Version 3.0 Getting Started Guide viiPrefaceHow to Use This GuidePurpose of ThisGuideThe

Page 50

DRAFTDecember 16, 2004 3:54 pm, GSG_Preface.fmPrefaceHow to Obtain More Informationviii Primer Express Version 3.0 Getting Started GuideExamples of th

Page 51

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-01.fmPrimer Express Software 3.0 Getting Started Guide 1Notes1Chapter 1Before You BeginBefore You BeginI

Page 52 - Part Number 4362460 Rev. B

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-01.fmChapter 1 Before You BeginIntroduction2 Primer Express Software 3.0 Getting Started GuideNotesIntro

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