
DRAFT
December 16, 2004 3:54 pm, GSG_Chapter_4-03.fm
Chapter 3 Designing Primers and Probes for Allelic Discrimination Assays
Overview
28 Primer Express Software 3.0 Getting Started Guide
Notes
Overview
About This
Chapter
This chapter provides information on using Primer Express
®
software to automatically
design primers and probes for allelic discrimination assays using default parameters. It
also includes information on how to manually design primers and probes to obtain
customized results.
Workflow
Figure 8. Allelic Discrimination workflow
YesNo
Assign a SNP Target
Find Primers and Probes
View Results
Primers and Probes
Found?
Order Primers
and Probes
Manually Design
Probe 1
Manually Design
Probe 2
Manually Design
the Primers
Desired
Primers and Probes
Found?
Edit Default
Parameters
Order Primers
and Probes
YesNo
Open or Create an Allelic
Discrimination Document
Load a DNA Sequence File
Allelic Discrimination Workflow
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