AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Manuel d'utilisateur télécharger pdf (Page 39)

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December 16, 2004 3:54 pm, GSG_Chapter_4-03.fm

Chapter 3 Designing Primers and Probes for Allelic Discrimination Assays

Automatically Designing Primers and Probes

32 Primer Express Software 3.0 Getting Started Guide

Notes

Figure 11. Primer/Probe Tab displaying candidate primers and probes

Evaluating the candidate primer and probe sets:

The Location section illustrates the location of the primers and probes within the line

sequence. The number above the line is the starting base; the number below the line is

the ending base. Note that you can also see the corresponding location of a selected

candidate Primer/Probe set in the Sequence tab.

In the sequence tab, the probe 1 will be highlighted in pink, probe 2 will be highlighted

in green (if probe 1 and 2 overlap, the overlap region will appear green), the forward

primer in blue, and the reverse primer in yellow (see “Figure 12. Probe annotations in

Sequence tab” on page 33). These default color designations can be changed by clicking

Tools > Opt ions. If you place your cursor over any of these annotations, a tool tip will

appear showing the name of the annotation (Probe, Forward Primer, Reverse Primers)

start and end locations, Tm and GC%.

1 2 ... 34 35 36 37 38 39 40 41 42 43 44 ... 51 52

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AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Manuel d'utilisateur Page 39