AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Manuel d'utilisateur télécharger pdf (Page 26)

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December 16, 2004 3:54 pm, GSG_Chapter_4-02.fm

Chapter 2 Designing Primers and Probes for Quantification Assays

Automatically Designing Primers for SYBR

Green Dye Assays

Primer Express Software 3.0 Getting Started Guide 19

Notes

Automatically Designing Primers for SYBR

Green Dye

Assays

Note: This procedure generates primers and TaqMan probes. However, only the primers

need to be ordered for SYBR

Green Dye assays. If desired, you can save the probe

sequence for future use in TaqMan assays.

Creating a

Quantification

Document

To create a new quantification document:

1. Select File > New. The New dialog box opens.

2. In the Type list, select Taq Man

MGB Quantification or TaqMan

Quantification.

3. Click OK.

The document window opens to the Sequence tab.

Loading a DNA

Sequence File

A sample sequence NM_002217, is located in the sample sequences folder within the

Primer Express folder. You can use this sample file to experiment with the software and

design your primers.

To load a sequence file:

1. Select Tools > Add DNA File ( ). Note you can also copy and paste or type

your sequence file in the Sequence tab.

2. At the Add DNA File dialog box, navigate to and select the desired file. For

information on the various file formats supported, see Primer Express Software

Version 3.0 Online Help.

3. Click Add. Primer Express software loads the nucleotide sequence from the file and

displays the sense strand in the Sequence tab (see “Figure 5. Sequence tab” on

page 20). The sequence serves as the starting point for primer design.

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AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Manuel d'utilisateur Page 26