AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Manuel d'utilisateur télécharger pdf (Page 37)

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December 16, 2004 3:54 pm, GSG_Chapter_4-03.fm

Chapter 3 Designing Primers and Probes for Allelic Discrimination Assays

Automatically Designing Primers and Probes

30 Primer Express Software 3.0 Getting Started Guide

Notes

Figure 9. Sequence tab

Note: If you select the Double-Stranded checkbox in the Sequence tab, both sense and

anti-sense strands will be displayed. However, primers and probes are designed using the

sense strand sequence only.

Assigning a SNP

Target

To assign a SNP target:

1. Highlight the SNP target site.

2. Select Edit > Annotate > SNP Target ( ) then select the variant for the SNP

site. To determine the variant to select, find the two possible variant bases for your

SNP, then click the code between the two bases. In the example sequence provided,

the SNP target is located at position 528 as a G/A variant, so click R, then OK (see

“Figure 10. Determining variant using SNP Target Tool” on page 31).

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AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Manuel d'utilisateur Page 37